SOPs & Guidelines

As every approach and every set up is different due to the sample, used method etc., there are of course not the guidelines.

But anyhow, to use a common strategy for all partners and to allow compareability of results please take a look on the SOPs recommended below...

In general:
Some rather general considerations concerning proteomics are to be found in Hamacher Meyer.pdf

Human body fluids:
SOPs according to the guidelines generated by Jens Wiltfang & Piotr Lewczuk, Erlangen-Nuermberg within the Dementia Network.

Checklist pre-analytical sample handling.pdf

Protocol for CSF collecting.pdf

Protocol for serum, plasma, blood collecting.pdf

Protocol for shipment.pdf

View of matrix box.pdf


Sample preparation 2D-PAGE:

SOP according to Jochim Klose's paper...

Preparation of Protein Samples from Mouse and Human Tissues for 2-D Electrophoresis.pdf


Human brain tissue:

Related to disease groups the Brain-Net Europe (organized by H. Kretzschmar, Munich) recommends the following procedures:

Dementia:
Dementia protocols (pdf, 17 k)

Depression and Schizophrenia:
Section protocol (pdf, 57 k) & Regions of interest (pdf, 55 k)

Motor Neurone Disease:
Motor neurone disease protocols (pdf, 25 k)

Neuro-degenerative Diseases:
Neuro degenerative disease protocols (pdf, 12 k)

Last Updated (Wednesday, 03 March 2010 12:06)